Thursday, December 19, 2019

Toxicity of Compound in the Zebrafish Bioassay Lab Report - 1

Essays on Toxicity of Compound in the Zebrafish Bioassay Lab Report The paper "Toxicity of Compound in the Zebrafish Bioassay" is a good example of a lab report on chemistry. For Dr. Pack, our UPenn gastroenterologist collaborator, to test the toxicity of compound 2 in the zebrafish bioassay, we provided Dr. Porter with the compound. At the end of the chemical reaction session, a certain research grouped challenged that they were in a position to isoflavone using AlCl3in dioxane. In this case, the group did not state any conditions for this reaction to take place. As such, I decided to optimize the reaction conditions starting with the model compound Chromanone. In this case, I mixed chromanone and AlCl3 in dioxane and then refluxed the content for 3 hours. Unfortunately, I did not obtain positive results and hence I was prompted to repeat the procedure. In this attempt, I decreased dioxane’s volume from 50 ml to 20 ml, used 10% of AlCl3, and I prolonged the reaction time from 3 hours to 12 hours. During the reaction, I monitored the proceeding using TLC. However, I did not obtain the intended products after several tests. In the third attempt, I changed most of the conditions and measured used in the first two trials. In this case, I used 0.500 gm of chromanone in 20 ml dioxane and 0.40 mg of AlCl3 which represents 10% of the starting material. I also reduced the frequency of monitoring to 2 hours. After the first two hours, the starting materials had completely disappeared. This indicated that I had optimized the TLC condition, by using different ratios of ethyl acetate and hexane. Then, I ran prep-TLC and I realized 4 different compounds. I then separated these 4 new products and conducted tests by NMR. However, the 4 compounds did not match the expected NMR spectrum of the desired product.My failure in these experiments prompted me to carry out research in journals and other materials. In this case, I came across some work discussing ring opining reaction on isoflavanones. The source has indicated all the conditions and the details about the experiment. However, the source suggests that I should use LiAlH4 which is a hazardous chemical. We had training with Dr. West on pyrophoric materials and now we are fully prepared to do the reaction.

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